South Carolina Gamecocks superfan Darius Rucker gave “Wagon Wheel” an R-rated upgrade during a concert this week … getting the whole crowd to chant “f*** Tennessee” in the middle of it — and it’s incredible.

The Hootie and the Blowfish frontman was part of the South Carolina women’s basketball championship party on Sunday night … and naturally, he had to put his passion for the ‘Cocks on full display.

In fact, when it came time to do his hit song, Rucker switched up the lyrics that go “But he’s a-headin’ west from the Cumberland Gap To Johnson City, Tennessee” … and instead, had the whole arena scream “f*** Tennessee at the top of their lungs to show hate for the Volunteers.

The moment is awesome … and is quite the proper way to celebrate South Carolina’s epic win over UConn earlier this month.

Nelly was also present for the free concert … so the night was truly one to remember.

Of course, we’ve seen stuff like this happen in the past … with Justin Bieber trolling fans in Montreal over his allegiance to the Toronto Maple Leafs.

At least this time around, Darius knew his audience!!